e8.5d小鼠神經(jīng)管來源的神經(jīng)上皮細胞micrornas的表達譜分析

1、華中科技大學碩士學位論文E8.5d小鼠神經(jīng)管來源的神經(jīng)上皮細胞microRNAs的表達譜分析姓名：陳海萍申請學位級別：碩士專業(yè)：康復醫(yī)學與理療學指導教師：黃曉琳20080501華 中 科 技 大 學 碩 士 學 位 論 文 II Analysis of microRNAs expression in neuroepithelial cell from neural tube of E8.5d

2、mouse Department of Rehabilitation and Medicine, Tongji Hospital Affiliated with Tongji Medical College, Huazhong University of Science and Technology Postgraduate CHEN Haiping Tutor Prof. HUANG Xiaoli

3、n Abstract Objectives: The microRNAs are some small non- coding transcripts of about 2 2 n tnucleotides which play important roles in developmental regulation. The aims of this study are to find

4、some mouse neural development related microRNAs, and to explore molecular mechanism of neurogenesis and provide theory basis and potential treatment target on spinal cord injury. Methods :E8.5d an

5、d E9.5d mouse neural tube were selected, the expression of Nestin were detected with immunohistofluorescence and Nestin positive cells were counted by Flow Cytometry（FCM）. microRNAs expression pro

6、filing in E8.5 mouse neural tube was analyzed by microarray method. Then the target genes of all these microRNAs were predicted by bio- information method (http://pictar.bio.nyu.edu, http://www.tar

7、getscan.org). Results: Almost all the neuroepithelial cells in E8.5d mouse neural tube were Nestin positive, while few cells in E9.5d mouse neural tube expressed Nestin; By FCM, the Nestin posit