夜行性昆蟲(chóng)的趨光行為及黑光燈對(duì)棉鈴蟲(chóng)體內(nèi)酶系的影響.pdf

1、華中農(nóng)業(yè)大學(xué)碩卜學(xué)位論文摘要燈光誘殺害蟲(chóng)是一個(gè)應(yīng)用廣泛而有效的方法，但燈光誘殺害蟲(chóng)的機(jī)理并沒(méi)有完個(gè)闡明，而且如何提高燈光誘殺害蟲(chóng)的效果尚待研究。為了進(jìn)一步提高燈光的誘殺效果，并從昆蟲(chóng)酶系的角度對(duì)燈光誘殺的機(jī)理進(jìn)行闡述。本論文對(duì)夜行性昆蟲(chóng)對(duì)不同波段的光波的趨光行為及黑光燈波段的光波對(duì)棉鈴蟲(chóng)成蟲(chóng)體內(nèi)酶系的影響進(jìn)行了初步研究，主要結(jié)果如下:1.夜行性昆蟲(chóng)對(duì)不同波段光波的趨性不同，(320nm390nm)、藍(lán)光燈波段(350nm490nm從大到

2、小依次為黑光燈波段)、紅光燈波段(500mn565nm)綠光燈波段(555nm610nm)、黃光燈波段(625nm670nm)，且黑光燈波段顯著大于其它四個(gè)波段。2.各波段對(duì)益蟲(chóng)的誘殺情況，從總數(shù)看，黑光燈對(duì)益蟲(chóng)的誘殺顯著大于其它四個(gè)波段，但從益害比看，綠光燈對(duì)益蟲(chóng)的選擇性最高。3.不同目昆蟲(chóng)對(duì)各波段光波的趨性:半翅目昆蟲(chóng)，無(wú)論從數(shù)量還是比值上，對(duì)黑光燈的趨性最強(qiáng)鱗翅日昆蟲(chóng)，在數(shù)量上，對(duì)黑光燈趨性最強(qiáng)，但從比值看，綠光燈對(duì)其選擇性最高鞘

3、翅目昆蟲(chóng)，在數(shù)量上，對(duì)黑光燈趨性最強(qiáng)，但從比值看，藍(lán)光燈對(duì)其選擇性最高雙翅目昆蟲(chóng)，在數(shù)量上，對(duì)紅光燈趨性最強(qiáng)，但從比值看，黃光燈對(duì)其選擇性最高同翅目昆蟲(chóng)，在數(shù)量上，對(duì)黑光燈趨性最強(qiáng)，但從比值看，黃光燈對(duì)其選擇性最高直翅目昆蟲(chóng)，無(wú)論從數(shù)量還是比值上，對(duì)黑光燈的趨性最強(qiáng)。4.棉鈴蟲(chóng)雌雄成蟲(chóng)之間對(duì)五個(gè)波段光波的行為反應(yīng)無(wú)顯著差異16羽化日齡的棉鈴蟲(chóng)成蟲(chóng)間對(duì)五個(gè)波段光波的行為反應(yīng)無(wú)顯著差異。5.暗適應(yīng)時(shí)間對(duì)棉鈴蟲(chóng)成蟲(chóng)的光行為反應(yīng)有影響，暗適應(yīng)1

4、5min與30min之間有顯著差異。6.五個(gè)光處理時(shí)間梯度對(duì)棉鈴蟲(chóng)雌成蟲(chóng)的過(guò)氧化氫酶(CAT)無(wú)影響。超氧化物歧化酶(SOD)，光處理45min的活力最高，極顯著高于其它處理15min30min60min間無(wú)顯著差異。谷膚甘肚S一轉(zhuǎn)移酶(GSTs)，無(wú)光處理的活力最高，極顯著高于其它處理，其它處理間無(wú)顯著差異。酸性磷酸醋酶(ApE)，無(wú)光處理的活力最高，極顯著高于45min60min的，但與15min30min的無(wú)顯著差異。梭酸酷酶(C

5、arE)，光處理15min的活力最高，極顯著高于其它四個(gè)處理。華中農(nóng)業(yè)大學(xué)碩」學(xué)位論文NocturnalinsectsphototacticbehaviortodiferentlightandtheefectsofBlacklightontheenzymeinbollwormHeicoverpaarmigeraAbstractInthethesisnocturnalinsectsphototacticbehaviorsunderdife

6、rentwavelengthlightandtheefectsofthemainwavelengthlightontheenzymeinbollwormHelicoverpaarmigeraexaminedandthemainresultsareasfollows:1.Theratioofphototaccticinsectsunderdiferentwavelengthlightwasdiferent.Andthemaxmumwasu

7、nderblacklightwavelength(320nm390nm).Nextwasunderbluelightwavelength(350nm490nm).Thethirdwasunderredlightwavelength(500nm565nm).Thefourthwasundergreenlightwavelength(555nm610nm).Andtheminimumonewasunderyellowlight(625nm6

8、70nm)wavelength.Theeficiencyunderblacklightwasthebestofallandwassignificantly(0.05)higherthantheotherfour.2.Beneficialinsectshaddiferentphototaxistodiferentlights.Theefectofblacklightonthebeneficialinsectswassignificantl

9、yhigherthantheotherlightswhilethegreenlightwasthemostefficientoneastotheratioofbeneficialinsectstopests.3.Insectfromdiferentorderhaddiferentphototaxistodiferentlight.TheinsectsinHemipteraphototactictoblacklightwerelarges

10、tinbothtotalandn..ioascomparedtootherlights.TheinsectsinLepidopteraweremostphototactictoblacklightwhilemorebeneficialinsectstogreenlight.TheinsectsinColeopteraweremostphototactictoblacklightwhilemorebeneficialinsectstobl

11、uelight.TheinsectsinDipteraweremostphototactictoredlightwhilemorebeneficialinsectstoyellowlight.TheinsectsinHomopteraweremostphototactictoblacklightwhilemorebeneficialinsectstoyellowlight.TheinsectsinOrthopteraweresimila

12、rtotheinsectsinHemiptera4.Wetestedbollwormsbehaviorunderlights.Therewasnosignificant(0.05)diferenceinthebehaviorbetweenmaleandfemale.Therewasnosignificant(0.05)atrierenceamongadultsaged16emergencyday.5.Thedarkadaptedtime

13、hadsomeefectonthephototacticbehaviorofbollworm.Andtherewassignificantdiferencebetween15minand30mindarkadapi.idtime.6.Thefiveilluminestime(Omin15min30min45min60min)wascarriedonfemalebollworm.Therewasnosignificant(0.05)efe

14、ctonCATinfemalebollwormamodiferentilluminetime.TheactivityofSODin45illumineng(0.01)higherthanothertreatmentsminuteswassignificantly，whiletherewasnosignificant(0.05)diferenceinISmin30minand60min.TheactivityofGSTswasstrong