新型復合乳酸菌制劑的初步研究.pdf

1、全文摘要新型復合乳酸菌制劑的初步研究全文摘要對乳酸乳桿菌、嗜酸乳桿菌、乳酸鏈球菌三株乳酸菌的抑菌特性和耐藥性進行了研究，依據微生態(tài)學原理，研制了三株菌和低聚異麥芽糖復合的益生菌制劑，并測定了該制劑的臨床藥效，以期為臨床提供優(yōu)良的產品。乳酸菌體外對大腸埃希菌的拮杭作用。將乳酸菌與大腸埃希菌體外共培養(yǎng)，觀察拮杭情況及乳酸菌濾液對大腸桿菌的抑制情況。結果顯示，等量同時間接種，96h內乳酸菌就將大腸埃希菌全部抑殺乳酸菌早24h接種，72h內將大

2、腸埃希菌全部抑殺大腸埃希菌早24h接種，在120h內也可全部被抑殺.三株菌按一定比例復合，會使拮抗的效果增強.乳酸菌的培養(yǎng)濾液對大腸埃希菌抑制實驗顯示，對大腸埃希菌的抑菌圈直徑均在13一15mm之間，乳酸菌培養(yǎng)濾液與同等PH乳酸相比，直徑略大，但差異不顯著。乳酸菌制劑中三株乳酸菌菌種的藥敏性及耐藥傳遞的體外試驗。結果顯示，本制劑中的三株乳酸菌對多種抗菌藥物有耐藥性，僅對氛帶西林和氛霉素有著不同程度的敏感性耐藥性傳遞的體外試驗結果提示，無

3、論是24h還是18次傳代試驗，鏈霉素的杭性未轉移給鏈霉素敏感株大腸埃希菌.低聚異麥芽糖和低聚果糖體外時乳酸菌的增殖和對大腸埃希菌的桔杭作用兩種低聚糖汁雛雞大腸埃希菌病的拮杭作用和對雛雞盲腸乳酸菌、大腸埃希菌的影響低聚異麥芽糖與乳酸菌的正交復合對禽大腸埃希菌病的預防作用。結果顯示:添加低聚糖組對乳酸菌有一定的生長促進作用，pH值明顯下降，但與對照組相比，差異不顯著低聚糖十復合益生菌組對大腸埃希菌的拮杭作用顯著，對雛雞盲腸乳酸菌的定植和大腸

4、埃希菌的拮杭作用，在5d后呈現(xiàn)顯著性低聚糖十益生菌兩個劑量組對禽大腸埃希菌病的治愈率為75%一90%，而對照組為功%。兩種低聚糖之間比較差異均不顯著.低聚異麥芽糖與復合益生菌正交復合預防禽大腸埃希菌病的試驗顯示，4g低聚異麥芽糖108個益生菌效果較好。復合乳酸菌對9日齡雛雞人工誘發(fā)禽大腸埃希菌病的臨床療效及對雛雞的生產性能和盲腸乳桿菌、大腸埃希菌的影響。結果顯示，對人工誘發(fā)禽大腸埃希菌病預防試驗，中高劑量治愈率達80%，藥物組70%一7

5、5，對照組存活率僅為40%，差異極顯著(P0.01)對禽大腸埃希菌病的治愈率為70%左右，與藥物組效果相當，對照組存活率僅為50%，但與藥物組相比，復合乳酸菌治療的存活率不及藥物對雛雞21d的增重實驗，平均增重較對照組提高7.5%，料肉比下降6.7%t檢驗差異顯著英文摘要PRELIMILARYSTUDYONMULTIM0DULATER0FLACTOBACILLUSABSTRACTInthispapertheantagonisticpro

6、pertyanddrugresistanceofthreeLactobacilluswerestudied.BasedonthemicroecologicalprincipleamultimodulatesofprobioticswhichincludingLactobacillusacidophilusLactobacilluslacti，StreptococcuslacticandIsomaltooligosaccharideswe

7、reresearched.InordertooferachoicenessproductionforclinicweinvestigatedclinicalefficacyofthemultimodulatesofprobioticsToinvestigatewhetherthereisantagonismofLactobacillustowardEscherichiacoliinvitroLactobacillusandEscheri

8、chiacoliwereculturedtogetherthenobservedthesurviveofEscherichiacoliandtheinhibitionoftheLactobacillusfiltrateonEscherichiacoli.TheresultsshowedthatInoculatedLactobacillusandEscherichiacoliatthesametimeandvolumesEscherich

9、iacoliwerekilledin96h.Escherichiacoliwerekilledin72hand120hrespectively，wheninoculatedLactobacillus24hearlierandlaterthanEscherichiacoli.TheantagonismefectofLactobacillustowardEscherichiacoliwillbeimprovedifthethreestrai

10、nswerecompoundedingivenproportion.TheexperimentofthefiltrateofLactobacillusinhibitingEscherichiacolishowedthatantimicrobialzonediametersofthefiltrateandlacticacidonEscherichiacoliwere1315mmandtherewerenosignificantdifere

11、ncesbetweenthefiltrateandlacticacidbuttheantimicrobialzonediametersoffiltrateshowedbigger.AntibioticsusceptibilityandconjugaltransferofstreptomycinresistanceinvitrofromLactobacillustoEscherichiacoliwerestudied.Theresults

12、howedthatthreeLactobacillusstrainswereresistedtomanyantibioticsonlysensitivetoampicillinandchloramphenicot.ThreeLactobacillusstrainsdidnttransfertheirresistanceofstreptomycintoEscherichiacoliwhichissusceptivetostreptomyc

13、inwhereasin24horinlongtimetestTheefectofmultiplicationofoligosaccharidestoLactobacillusandantagonismofoligosaccharidestoEscherichiacoliinvitrotheefectofpreventingfromaviancolibacillosisadhesionofLactobacillusandinhibitio

14、nofEscherichiacoliinchickensjejunumanddiferentdosagesofIsomaltooligosaccharidescompoundedwithdiferentdosageofLactobacillustopreventfromaviancolibacillosiswerestudied.TheresultshowedthatLABSweregrowingmoreandPHwerelowerin