無水乙醇碘油混合乳劑對兔VX2肌肉腫瘤作用的實驗研究.pdf

1、徐州醫(yī)學院碩士學位論文無水乙醇碘油混合乳劑對兔VX2肌肉腫瘤作用的實驗研究姓名：朱敏申請學位級別：碩士專業(yè)：影像醫(yī)學與核醫(yī)學指導教師：徐浩201203一徐州醫(yī)學院碩士學位論文———————————————————————————二—————二————————一一TheexperimentalstudyofEthanol—LipiodolmixedemulsiononrabbitVX2muscletumorAbstractObjectiv

2、eToevaluatetheinhibitoryeffectontheVX2muscletumorofrabbitwithpercutaneousethanol—lipiodolmixedemulsioninjectingMethodsAfterNewZealandwhiterabbitsweremadeforVX2muscletumormodelthe16rabbitsimplantedVX2tumortissue(32models)

3、weredividedinto4groups(4rabbitsineachgroup)randomly：normalsaline(GroupA)，lipiod01(GroupB)，ethanol(GroupC)andofethan011ipiodoi(1：1)mixedemulsion(GroupD)Accordingtotheabovegrouppercutaneousinjectionofthecorrespondingsubsta

4、ncesthedosefor2ml／eachtumorlociAfteroneweek，allanimalswerekilIedandtheeffectwasevaluatedThenecrosisoftumortissueswereusedforoilredOtoobservethedistributionoflipiodolundertheopticalmicroscopeThenecrosisoftumortissueswereo

5、bservedbyHEstainingundertheopticalmicroscopeandthetumornecrosiswerecalculatedTheultrastructuralchangesoftumorcellswereobsen，edundertheelectronmicroscopyResults(1)Thetumornecrosis：groupA(3131961)％、groupB(4617士1620)％、group

6、C(7501士601)％、groupD(8716士465)％，Tocomparethedifferencesweftstatisticallysignificantamongthefourgroups(P005)ThehighestofthetumornecrosisratewerefoundintheDgroup(2)Pathology：ComparedwithgroupA，butthemostseriousoftumorcellne

7、crosiswerefoundintheDgrouDThetumorcellshasshowedlargenecrosisandfragmentationofthenuclearoftumorcells(3)OilRedOstaining：GroupBandgroupDhasshowedthedistributionoflipiodolintheclearanceoftumorcellsafteraweek，Thelipiodolpar

8、ticlesofgroupDshowedmoredensedistribution(4)theelectronmicroscopy：asshownintheincreasesofperinucleargap，nuclearshrinkage，condensation，nuclearbreakdownandorganellesdisappearConclusions1Percutaneoustheinjectionoflipiodolma