晶體蛋白對離體培養(yǎng)的大鼠視網膜神經節(jié)細胞的作用研究.pdf

1、第三軍醫(yī)大學碩士學位論文晶體蛋白對離體培養(yǎng)的大鼠視網膜神經節(jié)細胞的作用研究姓名：王艷華申請學位級別：碩士專業(yè)：眼科學指導教師：王一20040501第三軍醫(yī)大學碩士學位論文2EffectofcrystallinesonsurvivalgrowthofratRGCsinvitroAbstractHowtoprotectsurvivalofRGCspromoteaxonalregenerationafterONinjuryhasbecomea

2、focusalloverthewld.NowithasbeenprovedthatlensinjurycanpromotesurvivalofRGCsaxonalregeneration.TheinductionoflensinjuryresultsinreleaseoflentogenicfactswhichpromotesurvivalofRGCsstimulateaxonalregenerationthroughouttheopt

3、icpathwayarriveatthethalamusatlast.Butwhat’reonearththelentogenicfactsIthasnotbeenanswereduntiltoday.Leonfoundthattherewerealotofmacrophagesintheretinaafterlensinjurythoughtthatactivatedmacrophagesplayedagreatrole.Howeve

4、rthere’stimediscrepancybetweenmacrophageinfiltrationaxonalregenerationaxonalregenerationcannotbeexplainedcompletelybytheeffectofmacrophage.Lensisatissuecontentingthehighestproteincomposedof66%water33%protein.Solublecryst

5、allins()makeup90%95%ofthetotalproteinofthelens.Asselfantigenscrystallinscanactivatemacrophagespromotetheirinfiltrationinlocaltissue.assmallheatshockproteinsalphaAalphaBtwoalphacrystallinsubunitofcrystallincaninhibittheac

6、tivationofcaspase3promotethesurvivalofretinalpigmentepithelialcellsphoteceptslensepitheliumcells.WhethercrystallinscanpromotethesurvivalofRGCsnotPurpose:Toestablishculturemethodevaluationtechniqueofretinalganglioncellsin

7、LongEvansratsinvitro.StudytheeffectofcrystallinsonsurvivalgrowthofratRGCsinvitrodiscusstherelationbetweencrystallinsactivatedmacrophagesabouttheireffectonratRGCsinvitro.Therebyquesanewmethodfthetreatmentofopticnerveinjur

8、yprovideexperimentalbasisontheregenerationofopticnerveinjury.Methods:CrystallinswereextractedfromLongEvansratsculturemediaofmacrophageswerepreparedbyculturingenterocoeliamacrophagesofLongEvansratsf24h.RGCsinLongEvansrats