連云港近海岸海洋沉積物中微生物群落結構與功能的初步研究.pdf

1、江南大學碩士學位論文連云港近海岸海洋沉積物中微生物群落結構與功能的初步研究姓名：朱廣超申請學位級別：碩士專業(yè)：微生物學指導教師：李華鐘王淑軍20090801AbstraetAbstractLianyungangisallimportantportcityofChinaEnvironmentpollutionbecamemoreandmoreserious、析n1theeconomicdevelopmentinrecentyearsInt

2、hisstudythetypeofpollutantandthedegreeofpollutionin80samplesfromthreepillars(Z01，Z02，Z03)sampledfromdifferentinshoreareaswereanalyzed，theStnlCtureandthefunctionofthemicrobialcommunitywerestudied，thebacterialstrainswereis

3、olatedTheresultofmetalelementsanalysisshowedthatallthesamplesfromthreepillarswerepullutedwitharsenicandstannummoderatelyenrichedSimilarresultwerealsofoundwithbariumandzirconiuminZ01copperinZ02andbariuminZ03Themetalelemen

4、tsinthesedimentmainlyexistedintheformofresidue，followedbythestateofFeMnoxideandcarbonatecompound，thelessexistenceiStheexchangeablestateThemicrobialcommunitys缸1lctl鵬andthefunctiongenesofmalinesedimentsof22samplesfromthree

5、pillarswerestudiedbythemicroarrayTheresultshowedthatmicrobialcommunity咖ctIlreandthefunctiongenesweresignificantlydifferentamongthreepillarsZ01hasgreatestdiversityhavingmicrobialsfrom10differentgenerasuchasActinobacter，Ps

6、eudomonadalesandSOonForZ02andZ03，eachhasmicrobialsbelongingto6differentgenerasuchasBdellovibrionales，DesulfovibrionalesandSOonTheclusteringanalysisindicateddifferentsamplesofthesamepillarhadthesimilarfunction111ecanonica

7、lcorrespondenceanalysisonrelationshipbetweenthecompositionandfunctionofmicrobialcommunityandpollutionsbyanalyzingthecompositionanddynamicsofmicrobialcommunityandtheparametersofmainpollutionsfrommarinesedimentswereperform

8、edandtheresultsshowedthatthemicrobialcommunitystructureandfunctionweresignificantlyinfluencedbyzirconium，petroleum，conductivityandarsenic111eresultmaybenefittobioremediationofmarinepollutionTotalof51strainswereobtained，r

9、esultofphysiologicalandbiochemicalcharacteristicdeterminingand16SrDNAgenesiringappraisalindicatedtheybelongto17generasuchasBacill淞BrevundimonasandSOonBacilluscoveredthemajorityofisolatesAstrainresistanttocopperlcadandchr

10、omiumwasisolatedfrom51strainsseparatedfrommarinesedimentsThestrainwasidentifiedasArthrobacterIt’SbiologicalcharacteristicswerestudiedDifferentconcentrationsofheavymetaliOIlSextendedthelagphagetovariousdegreesThewaterishs

11、trainsCanadsorbcopper(II)andlead(II)by7722％，9543％，respectivelybutCannotadsorbchromium(VI)，andthischaracteristicswerestableafter10generationsKeywords：Marinesediment，Microbialcommunitystruture，functionalgenes，MicroarrayHea